Cloning and Expression Gene in HD34 Beer Yeast
نویسندگان
چکیده
Alcohol Dehydrogenase (ADH) gene was isolated from Bacillus subtilis by PCR amplification. The 1.1kb amplified fragment was confirmed to be the ADH gene by DNA sequencing. The gene was inserted into expression plasmid pYC6/C7, transformation HD34 beer yeast, as well as some transformants with ADH activity were obtained. [Nature and Science, 2004,2(2):64-66]
منابع مشابه
CLONING AND EXPRESSION OF HUMAN IFNα2B GENE IN SACCHAROMYCES CEREVISIAE
Interferon is a protein secreted by eucaryotic cells following stimulation by viruses, bacteria, and many other immunogenes. Recent medical studies indicate that interferons have effective role in the treatment of virus infections, immunodeficiency and certain types of cancer such as hairy cell leukaemia (HCL). The aim of the present study is to apply yeast strain for secreting human IFNα2b fol...
متن کاملIsolation, Subtype Determination, Cloning and Expression of HBsAg Gene from an Iranian Carrier in Saccharomyces cerevisiae
The Hepatitis B Surface antigen ( HBsAg) gene was isolated from an Iranian HBeAg positive carrier by PCR. The gene was cloned in pUC19 for sequencing and pYES2 for expression in Saccharomyces cerevisiae, which pNF1 and pDF3 constructs were made respectively. The sequencing data showed that the isolated HBsAg gene shared more than 90% homology with the ayw subtype. The pDF3 was transferred into ...
متن کاملCloning of fusion (F) protein gene of peste des petits ruminants virus (PPRV) in secretory Pichia pastoris vector
With advent and development of DNA recombinant technology and advantages of p. pastoris expression system, fusion (F) protein of PPRV expression, because of effective immunodominant role could be an appropriate candidate for production of recombinant vaccine against PPR disease. In this study, F gene of PPRV Nigeria 75/1 strain (1637 bp) was amplified using RT-PCR and purified. It was then cl...
متن کاملIncreased Acetate Ester Production of Polyploid Industrial Brewer’s Yeast Strains via Precise and Seamless “Self-cloning” Integration Strategy
Background: Enhancing the industrial yeast strains ethyl acetate yield through a precise and seamless genetic manipulation strategy without any extraneous DNA sequences is an essential requisite and significant demand. Objectives: For increasing the ethyl acetate yield of industrial brewer’s yeast strain, all the ATF1 alleles were overexpressed t...
متن کاملThe effects of dietary administration of non alkohalic beer concentrate on expression of genes related to growth and skin mucus immunity in common carp (Cyprinus carpio)
In this study the effect of non alkohalic beer concentrate was investigated on the expression of growth hormone (GH), insulin growth factor1 (IGF1) genes and skin mucus immunity (Ig and ALP) in common carp. 180 pieces of common carp with average weight of 12.5 ± 2 g, were fed experimental diets supplemented with different levels of non alkohalic beer concentrate (0 [control], 0.5, 1 and 2%) (4 ...
متن کامل